Project

Part:BBa_K2805004

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


LOV2 -> mRuby

This part is an mRuby reporter backed by a C120op-based promoter site, inducibly controlled by a LOV2 light-inducible TF. Under blue (450-490 nm wavelength) light, an organism with this part will generate a red fluorescent protein. This part is optimized for use in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1086
    Illegal BglII site found at 369
    Illegal BglII site found at 2342
    Illegal BamHI site found at 126
    Illegal BamHI site found at 3239
    Illegal XhoI site found at 3074
    Illegal XhoI site found at 3248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1406
    Illegal NgoMIV site found at 2644
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None